WebNov 18, 1996 · The Thiobond expression system was used to produce N-, P-, C- and S-domains of C1qR/ CaR (representing the N-terminal region, the proline-rich central region, the C-terminal region, and a region spanning the intersection of the N- and P-domains (Table 1)). Webwas purified using Thiobond resin and enterokinase was used to immunize rabbits. The IgG fraction was obtained using a protein A column (Amersham Pharmacia Biotech, Buckinghamshire, UK). Western blotting The specificity of the antibody was confirmed by Western blotting using whole rat testis samples and a Leydig cell line (LC540, kindly …
Sulfhydryl Regulation of L-Selectin Shedding: Phenylarsine Oxide ...
WebJan 1, 1998 · The bacterially expressed nebulin fragments 7a and 8c were affinity purified with ThioBond resin (Invitrogen Corporation), and all procedures were based on instructions by the manufacturer, with modifications as specified below. Cell pellets from 3 h postinduced cultures were disrupted in 10 mM imidazole (pH 7.0). WebS-protein (104 aa, ribonuclease A minus S-tag peptide sequence) modified resin affinity purification. RNAse S assay possible for quantitative assay of expression levels. ... (ThioBond) resin. His-patch thioredoxin: 109 aa (11.7 kDa) N, C: Purification and enhanced expression: Use of His-patch modified thioredoxin for IMAC purification . 84. Lu ... asuransi purna jabatan direksi bumn
Tribobond™ 15 high temp. coating
WebAfter centrifugation for 15 min at 10,000 × g, the supernatant was collected and incubated with 2 ml of ThioBond™ resin (Invitrogen), activated as indicated by the supplier, for 60 … WebFind many great new & used options and get the best deals for 1/6 Custom Resin Christopher Lee Francisco Scaramanga James Bond Unpainted Head at the best online … WebAffinity purification with phenylarsine oxide-modified (ThioBond) resin. His-patch thioredoxin. 109 aa (11.7 kDa) N-,C-Purification and enhanced expression . Use of His-patch modified thioredoxin for IMAC affinity purification [84]. lacZ (β-Galactosidase) 116 kDa. N-,C-Purification. Purification using p-amino-phenyl-b-D-thiogalactoside ... asi mfg