Phenyl beads 6ff
WebJun 4, 2024 · 肝素-琼脂糖凝胶6FF注意事项: 1、 该凝胶从冷室或冰箱中取出后*好在室温下缓慢振摇恢复到室温,然后再装柱,以免产生气泡影响柱效。 2、 吸附:20-50mM,pH7.4-8.0的缓冲液,为避免离子交换的干扰,缓冲液中可以适当加50mM-100mM的NaCl,,*常用的为Tris-HCl缓冲液。 3、 洗脱:可以用pH7.4-8.0的缓冲液加0.2-2M进行阶段或者线性洗脱 … WebPhenyl Sepharose 6 Fast Flow (high sub). Matrix Highly cross linked agarose 6% Type of ligand Phenyl: R-O-CH 2-CH(OH)-CH 2-O-C 6 H 5 Bead form Rigid, spherical, macro porous …
Phenyl beads 6ff
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WebThe following table shows the characteristics of Phenyl Sepharose 6 Fast Flow (low sub and high sub) . Matrix Cross-linked agarose, 6% , spherical Type of ligand Phenyl: R-O-CH2-CH(OH)-CH2-O-C6H5 Particle form Rigid, spherical, macro porous Particle size, d50V 1 ~ 90 µm Ligand concentration ~ 25µmol phenyl/mL resin (low sub) WebPhenyl Beads 6FF(Low Sub) 和Phenyl Beads 6FF(High Sub)分别是低取代的芳香族疏水层析介质和高取代的芳香族疏水层析介质,其中苯基通过不带电、化学性质稳定的醚键连接至高度交联的6%琼脂糖微球上。可根据不同物质的分离要求、分离效率和结合载量不同来选择高取 …
WebPhenyl Sepharose 6 Fast Flow is composed of cross-linked, 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two … WebHotline service:0519-83820242 Technical support:0519-83736881. Home; Products Menu Toggle. Protein purification; Nucleic acid purification; Antibody purification
WebMay 28, 2024 · The BET family of proteins consists of BRD2, BRD3, BRD4, and BRDt. Each protein contains two distinct bromodomains (BD1 and BD2). BET family bromodomain … WebPhenyl Sepharose 6 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Phenyl Sepharose 6 Fast Flow is composed of cross-linked, 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages.
WebPhenyl Sepharose 6 Fast Flow is composed of cross-linked, 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. …
http://wolfson.huji.ac.il/purification/PDF/HCIC/AMERSHAM_HisTrapHICcolumns.pdf domino\u0027s pizza saint john nbWeb第一步.硫酸铵沉淀粗纯蛋白。 第二步.离子交换层析纯化蛋白。 第三步.分析蛋白的亲疏水性,蛋白亲水性较强,尝试疏水层析纯化提高纯度。 第四步.凝胶过滤层析提高纯度。 第五步.SEC-HPLC检测。 您可能对以下内容感兴趣: 凝胶过滤层析常见问题解析 蛋白纯化服务 常用蛋白标签对比 无标签蛋白纯化相关 •蛋白纯化服务 •蛋白表达服务 蛋白纯化相关服务目录 … domino\u0027s pizza sandwich menuhttp://biobasic-asia.com/wp-content/uploads/2024/01/Phenyl-Beads-6FF-High-Sub.pdf domino\u0027s pizza salem nhWeb90 µm. Binding capacity (mg /ml) > 40 mg. Approx. 40 mg. > 10 mg. Application. The high protein binding capacity and the small bead size ensures high-resolution and minimal sample dilution. The flow properties of the highly cross-linked agarose matrix make it an excellent choice for purification scale-up. domino\u0027s pizza salem indianahttp://www.g-clone.com/newsitem/278171832 qr pad\u0027sWebPhenyl Sepharose®6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. … domino\u0027s pizza rustburg vaWebPhenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources. Proteinases belonging to the serine, aspartate and metallo mechanistic classes were effectively retained by the ... domino\u0027s pizza salem il