Honenpak
WebGlyScope Honenpak C18* (for ABEE Labeling Kit) (Cat. No. JOM-J715) *Specially-treated Alkali-proof silica filler is used for Honenpak C18. 4. Solvent for HPLC analyses … Weband a Honenpak C18 column (Honen Co., Tokyo, Japan) according to the manufacturer’s directions. Preparation of the GMP–XOS conjugate. The GMP– XOS conjugate was prepared by the Maillard reaction. GMP (1.0g) and XOS (1.6g) were dissolved in distilled water (100ml) and lyophilized. The mixture was incubated at 50 C at a relative humidity of ...
Honenpak
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WebThe ABEE-derivatized sugars were eluted from a Honenpak C18 column at 45°C with 0.02% trifluoroacetic acid containing 10% acetonitrile at a flow rate of 1.0 ml/min. Arabinose (Ara) was used as an ... WebJan 16, 2004 · ABEE-converted monosaccharides were analyzed by reversed-phase high performance liquid chromatography (HPLC) with a Honenpak C18 column (75 mm×4.6 mm i.d.) (Seikagaku) . Samples were applied to the column at a flow rate of 1.0 ml/min with 0.2 M potassium borate buffer (pH 8.9) containing 7% acetonitrile as a solvent at 45°C and …
WebMay 21, 2007 · Monosaccharides and oligosaccharides were labeled with ABEE by using an ABEE labeling kit (Seikagaku Corporation, Tokyo, Japan), and the ABEE-labeled saccharides were separated on an ODS column (Honenpak C 18, 75 mm × 4.60 mm, Seikagaku Corporation, Tokyo, Japan) by HPLC (Shimadzu LC-10; Shimadzu, Kyoto, … WebMar. Drugs 2011, 9 717 performance liquid chromatography fitted with a reversed-phase column (Honenpak C18, 75 mm × 4.6 mm I.D.) and fluorometer (Ex. 305 nm, Em. 360 nm). , D
WebNov 2, 2009 · Q-mucin was hydrolyzed by 4 M TFA at 100 °C for 3 h, and the monosaccharides thus obtained were N-acetylated, labeled with ABEE, and analyzed by HPLC with a Honenpak C18 column (J-Oil Mills Inc., Tokyo, Japan) in accordance with the supplier’s instructions. 7, 8 A mixture of l-arabinose, l-fucose, d-galactose, d-glucose, d … Web製品名 製品コード GlyScope シアル酸分析キット ABEE Labeling Kit Plus S J711 本製品は、コスモ・バイオ株式会社から販売しております。. 詳しくは 操作のながれ 糖タンパク質、糖脂質、オリゴ糖、糖鎖など 糖分析 糖のグリコシド結合を 切断し、遊離します ...
Web10. GlycoScope Honenpak C18 column (4.6 mm x 75 mm) (COSMO BIO, catalog number: JOM-J715-1PC) 11. Filter paper (Munktell & Filtrak GmbH, catalog number: 113053) 12. …
Web“Honenpak c18” Glyscope Lectin staining kit “Sensor Plus ” Labelled Lectins (iotin –FIT HRP) Agarose Lectins Glyscope column for sugar analysis “Honenpak c18” Agarose Lectins Lectin chromatography columns for HPL Glyscope column for sialic acid analysis “Serotonin-HPL olumn” I want to: quotes about being independent womanWebCosmo Bio USA provides quality life science products from leading Japanese manufacturers, to laboratories, research institutes, life-science education and testing … quotes about being in good companyWebGlyScope Honenpak C18 (for ABEE Labeling Kit) is useful for not only physiological and functional studies, but also research on complex carbohydrate sugar chains. Features. … shirley lanham elementary school atsugi japanWebAug 1, 2007 · then analyzed using a Honenpak C18 column (4.6 mm i.d. × 75 mm) from J-Oil Mills Inc., 0.2 M potassium borate buffer (pH 8.9) containing 7% CH 3 CN, and a standard kit from J-Oil Mills Inc ... shirley lanham elementary schoolWebOct 18, 2013 · To compare the neutral sugar compositions of native and desulfated ascophyllans, the acid-hydrolyzed native and desulfated ascophyllans were applied to HPLC analysis with GlyScope Honenpak C18 column. The elution profiles of acid-hydrolyzed native and desulfated ascophyllans are shown in Figure 1 A and B, respectively. quotes about being informedWebSep 16, 2024 · Samples were separated on an ODS column (GlyScope Honenpak C18 for ABEE Labeling Kit, 75 mm × 4.6 mm, particle size 3 μm; J-Oil Mils, Inc.) using a mobile phase consisting of 0.2 M potassium borate buffer at pH 8.9 and acetonitrile (93:7 v/v) at a flow rate of 1.0 mL/min at 30°C and monitored fluorometrically at excitation/emission ... quotes about being injuredWebFig. 2 HPLC profile of ABEE-labeled CPS-1 on a Honenpak C18 column. Each peak was monitored using a fluorescence detector (Ex.305 nm, Em.360 nm) Table 1 Methylation … quotes about being in healthcare