Bodipy-c11 staining
WebDetection of Ferroptosis by BODIPY™ 581/591 C11 Ferroptosis is a distinctive form of regulated cell death that is driven by lethal accumulation of lipid peroxides in plasma … WebMar 10, 2024 · BODIPY-C11 indicates the production of reactive oxygen species (ROS) in a lipophilic environment through a change in the fluorescence of the probe; it is sensitive to reactive species formed from hydroperoxides, but not to hydroperoxides themselves (Drummen et al., 2002 ).
Bodipy-c11 staining
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WebC11-BODIPY(591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single cells. First, staining conditions were optimized by exploring the capability of the dye to stain algal cells under increasing cell and dye concentrations and different ... WebBODIPY 581/591 C11 can be used for study lipid peroxidation and antioxidant properties in living cells, or detect ferroptosis by reaction with hydroxyl radicals. ... Cell staining 2.1 Cell preparation: For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. ...
WebApr 6, 2024 · C11-BODIPY dye (10 µM; D3861; Thermo Fisher Scientific, Waltham, MA, USA) was used for lipid ROS staining according to the manufacturer’s instructions, with cumene hydroperoxide as a positive control. Briefly, stably transfected or parental 786-O and Caki-1 cancer cells were collected after treatment with URB597 and RSL3 singly or … WebMar 16, 2024 · Here, we describe a BODIPY-based dye that switches on fluorescence when absorbed by the cell membrane and turns off fluorescence in aqueous medium. The dye exhibits excellent contrast immediately after cell staining and does not require any wash-off for fluorescence cell imaging and flow cytometric experiments.
WebC11 C5 C1C12 Time (min) RFU Figure 7.2 FACS-based fatty acid uptake assays with different BODIPY-LCFAs. Mature 3T3-L1adipocyteswereincubatedwith2 mMofeitherBODIPYC15(Cat.#3821),BODIPY C11 (Cat. # 3862), BODIPY C12 (Cat. # 3822), BODIPY C1C12 (Cat. # 3823), or BODIPY C5 (Cat. # 3834) for the indicated … WebAug 12, 2016 · Lipid ROS was measured by C11-BODIPY staining coupled with flow cytometry. Full size image. Given that both autophagy and NCOA4 are involved in …
WebDec 20, 2024 · (C) Lipid ROS was determined by BODIPY C11 staining coupled with flow cytometry. (D) FH mutant cancer cells recovered and proliferated after CC starvation. Cells were treated with CC starvation for 24 hr (UOK262 and UOK262+WT FH) or 60 hr (UOK268 and UOK268+WT FH), then cells were incubated in full DMEM medium for 3 days.
WebOct 27, 2024 · C11-BODIPY resides in lipophilic membrane structures where it can be oxidized by reactive enzymatic and/or non-enzymatic pro-oxidant intermediates, … dcfs advocacy lineWebThe staining method of lipid peroxidation sensor C11-BODIPY was carried out as described by [21]. In brief, C11-BODIPY (581/591) (Thermo Fisher) stock solution was prepared in ethanol to... dcf safety agreement pdfWebSep 3, 2024 · For lipid hydroperoxides measurement, MEFs were treated as indicated for 8 h and lipid hydroperoxides was determined by BODIPY C11 staining coupled with flow cytometry. Glc Glucose; erastin, 10 μM. geforce 2060 super treiberWebJan 15, 2024 · 3.2 BODIPY-C11 Staining 1. Power on and warm up the FACS machine. Also, warm up the growth media and HBSS. 2. Six hours after treatment, take out the 6 … geforce 2060 rtx treiberWebApr 12, 2024 · a Representative images of lipid peroxidation in treated and untreated control and PKAN cells using BODIPY® 581/591 C11 staining. Control cells treated with Luperox® (500 μM) for 15 min were used as a positive control of mitochondrial lipid peroxidation. Scale bar = 15 μm. b Fluorescence quantification of oxidized form of BODIPY® C11. dcf safety standards wisconsinWebOct 14, 2024 · The accumulation of LC3II (faster migrating form) is indicative of the induction of autophagy. (R) Indicated MEF cells were treated with 1 μmol/L erastin alone or with autophagy inhibitor (BAF, 20 nmol/L; CQ, 50 μmol/L) for 12 h, cell death was quantified by PI staining; lipid ROS level was assessed by BODIPY C11 coupled with flow cytometry. dcf safe sleep courseWebAug 12, 2016 · Ferroptosis is an iron-dependent form of regulated necrosis. It is implicated in various human diseases, including ischemic organ damage and cancer. Here, we report the crucial role of autophagy, particularly autophagic degradation of cellular iron storage proteins (a process known as ferritinophagy), in ferroptosis. dcfsa family limit